A pilot study of estrogen's effects on bronchial myocyte adhesion molecule expression.

摘要: We examined the effects of estrogen on tumor necrosis factor α (TNF-α)-induced expression intracellular adhesion molecule (ICAM-1) and vascular (VCAM-1) in cultured human bronchial smooth muscle cells (BSMC). Experiments were performed triplicate T-75 tissue culture flasks containing normal BSMC. Four experiments carried out: untreated BSMC (control); TNF-α 1000 U/ml stimulation BSMC; forskolin 5 μM before estradiol 30 Cyclic adenosine monophosphate was measured by a commercially available radioimmunoassay kit. Cell ICAM-1 VCAM-1 quantified flow cytometry. Incubation with for 24 hours elicited 27-fold increase basal 2-fold (p>0.05). μM, 1 hour TNF-α, decreased TNF-α-induced 62% slightly 17%. The incubated 21%; remained unchanged found trend toward inhibition TNF-α-stimulated pretreatment estradiol. However, due to large variability within cell model, statistical significance not reached.