Four distinct classes of proteins as interaction partners of the PABC domain of Arabidopsis thaliana Poly(A)-binding proteins

摘要: Poly(A)-binding proteins (PABPs) play an important role in the regulation of translation and control mRNA stability eukaryotes, their functions are known to be essential many organisms. PABPs contain a highly conserved C-terminal segment termed PABC domain. The domain from human PABP interacts with PAIP1, PAIP2 RF3 via its PAM2 motifs. These interactions for modulating translation. Arabidopsis has eight PABPs, unexpectedly large number comparison other eukaryotes whose genomes have been sequenced. Six In this work, we identified PABC-interacting Arabidopsis. Two proteins, which named CID1 CID7, were initially isolated two-hybrid screen, eleven more predicted present proteome rice proteome. Among 24 PAM2-containing set, observed diversity modules intriguing function, ranging acidic regions similar PAM1 motif found PAIP1 PAIP2, domains such as small MutS-related domain, Lsm Ataxin-2, RNA recognition motifs (RRMs). We suggest that may evolved provide plants greater flexibility metabolism specific transcripts. also two genes, PAB2 (ubiquitously expressed) PAB5 (expressed reproductive tissues), viability, suggesting each vital function.