Regeneration and innervation in cultures of adult mammalian skeletal muscle coupled with fetal rodent spinal cord.

摘要: Abstract In a collagen-substrate culture, long strips of teased adult skeletal muscle fibers oriented toward ventral-root nerve fetal rodent spinal cord explant will regenerate rapidly as the neural outgrowth makes contact with muscle. Trophic enhancement early regenerative capacity is relatively nonspecific and can also be produced by contacts variety nonneural cells. Myotubes neurally deprived regenerate, show fibrillatory contractions, develop transient cross striations at their peak development in vitro, but soon afterwards, vivo, they atrophy. Further differentiation, maturation, long-term maintenance require innervation, vitro well vivo. culture this dependent upon organotypic both central peripheral network complex. Although electrophysiologic studies that neuromuscular transmission occur second week after coupling, junctional structures are primitive definitive loci cholinesterase activity not discernable (at light microscopic level) until about later. During following weeks gradual maturation motor endplate structure occurs, including increased complexity terminals postsynaptic specializations, illustrated silver impregnation, staining, other histologic techniques. The ordered progression regeneration through innervation quite comparable to basic aspects Organotypic cultures provide, therefore, reliable model system for some mechanisms underlying restoration functional relations which may difficult analyze situ.