Expression of the Duchenne's muscular dystrophy gene in cultured muscle cells.
作者： A A Lev , C C Feener , L M Kunkel , R H Brown
DOI: 10.1016/S0021-9258(18)47660-0
关键词:
摘要: A partial cDNA clone for the Duchenne's muscular dystrophy (DMD) locus was used to investigate expression of this in human muscle vitro. Hybridization a 14-kilobase RNA transcript demonstrated both fetal and mature skeletal four lines cells culture. The DMD not detected cultured outside lineage. In cells, gene evident only myotubes before after innervation with mouse spinal cord. Primary cultures myoblasts did show presence prior fusion form myotubes. An vitro model is potentially an excellent system which factors controlling normal how altered muscle.
sci-hub.st 参考文章(25)
J B Jaynes, J S Chamberlain, J N Buskin, J E Johnson, S D Hauschka, Transcriptional regulation of the muscle creatine kinase gene and regulated expression in transfected mouse myoblasts. Molecular and Cellular Biology. ,vol. 6, pp. 2855- 2864 ,(1986) , 10.1128/MCB.6.8.2855
B. R. Ransom, E. Neale, M. Henkart, P. N. Bullock, P. G. Nelson, Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties Journal of Neurophysiology. ,vol. 40, pp. 1132- 1150 ,(1977) , 10.1152/JN.1977.40.5.1132
Anthony P. Monaco, Corlee J. Bertelson, Chris Colletti-Feener, Louis M. Kunkel, Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy Human Genetics. ,vol. 75, pp. 221- 227 ,(1987) , 10.1007/BF00281063
Rose Yasin, Gisela Van Beers, K.C.E. Nurse, S. Al-Ani, D.N. Landon, E.J. Thompson, A quantitative technique for growing human adult skeletal muscle in culture starting from mononucleated cells Journal of the Neurological Sciences. ,vol. 32, pp. 347- 360 ,(1977) , 10.1016/0022-510X(77)90018-1
G.J.B. van Ommen, J.M.H. Verkerk, M.H. Hofker, A.P. Monaco, L.M. Kunkel, P. Ray, R. Worton, B. Wieringa, E. Bakker, P.L. Pearson, A physical map of 4 million bp around the Duchenne muscular dystrophy gene on the human X-chromosome Cell. ,vol. 47, pp. 499- 504 ,(1986) , 10.1016/0092-8674(86)90614-8
F.E. Stockdale, H. Holtzer, DNA synthesis and myogenesis Experimental Cell Research. ,vol. 24, pp. 508- 520 ,(1961) , 10.1016/0014-4827(61)90450-5
Rose Yasin, Frank S. Walsh, David N. Landon, Edward J. Thompson, New approaches to the study of human dystrophic muscle cells in culture Journal of the Neurological Sciences. ,vol. 58, pp. 315- 334 ,(1983) , 10.1016/0022-510X(83)90092-8
P. Nelson, C. Christian, M. Nirenberg, Synapse formation between clonal neuroblastoma X glioma hybrid cells and striated muscle cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 123- 127 ,(1976) , 10.1073/PNAS.73.1.123
Hans Lehrach, Don Diamond, John M. Wozney, Helga Boedtker, RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. ,vol. 16, pp. 4743- 4751 ,(1977) , 10.1021/BI00640A033
Edith R. Peterson, Stanley M. Crain, Regeneration and innervation in cultures of adult mammalian skeletal muscle coupled with fetal rodent spinal cord. Experimental Neurology. ,vol. 36, pp. 136- 159 ,(1972) , 10.1016/0014-4886(72)90142-2