A new procedure for extraction and measurement of soluble sugars in ligneous plants

摘要: An original modification of an analytical procedure has been developed to obtain purified extracts for sugar measurement in different parts ligneous plants by high-performance liquid chromatography. Extraction was performed at 4°C the presence water, methanol and chloroform. Only a representative part alcohol extract (methanol/water) analysis. internal marker such as maltotriose, added during extraction, makes it possible estimate loss correct concentrations. Compared with more standard method based on total soluble this new is precise. The use calibration standards shows good linear progression but does not guarantee accuracy absence reference or plant powder standard. It robust which can tolerate experimental variations without affecting results. high recovery rate (90%) maltotriose proves quality concentrations acceptable limits. Une de analytique est developpee dans cette etude pour obtenir des extraits purifies l'analyse sucres differentes parties plantes ligneuses par chromatographie HPLC. L'extraction realise en d'eau, et chloroforme. Seule une partie l'extrait alcoolique (methanol/eau) purifiee l'analyse. Un marqueur interne comme le ajoute pendant l'extraction, permet d'evaluer la perte sucre corriger les