An approximate 'steady state' condition in the ultracentrifuge

摘要: An analysis is developed for two-component systems in a modified steady-state condition which may be attained the ultracentrifuge. The method depends on sharpening effect generally occurs across moving boundary, because sedimentation coefficients increase with dilution; this counteracted by diffusion and, accordingly, and spreading effects made to balance proper selection of experimental conditions. Owing radial dilution inhomogeneous field, true steady state cannot occur, but certain characteristics boundary do become independent time. With multi-component yield single diffusional augmented heterogeneity: thus state, if attainable, can occur only when gravitational field increased also. When such are analysed terms equations valid systems, deviations from homogeneity manifested as difference between apparent coefficient (i.e. coefficient) coefficient. has been tested application purified protein polydisperse mucopolysaccharide. It shown that an approximately readily attainable under conditions, provides sensitive means detecting impurities characterizing heterogeneity, although 2-component not adequate interpret fully behaviour very systems.