An immunohistochemical study of the pulpal nerve supply in primary human teeth: evidence for the innervation of deciduous dentine.

摘要: The innervation of pulp and dentine was studied in fully formed human deciduous teeth using antibodies to calcitonin gene related peptide (CGRP). Freshly extracted healthy were divided, fixed, demineralised, cryosectioned treated with CGRP which then labelled horseradish peroxidase. Bundles nerve fibres passed from the apex root coronal region where a subodontoblast plexus formed. In cervical half some branched away main bundles supply both odontoblast layer dentine. Branches also reached Most terminated layer. areas marginal nerves observed between odontoblasts predentine; intratubular arose either this or directly pulp. crown more densely innervated than that root. one third had most followed by horn middle third. occurred regions present. Although many tubules contained single filament complex patterns termination observed. maximum penetration fibre into 125 microns. pattern shows similarities permanent dentition but among differences is high density dentinal region. latter point correlates clinical impression greater sensitivity experienced patients during invasive procedures performed without anaesthetic area.