Impacts of Molecular Diagnostic Technologies on Plant Disease Management

摘要: Detection and diagnosis of plant viruses has included serological laboratory tests since the 1960s. Relatively little work was done on detection pathogenic bacteria fungi prior to development ELISA monoclonal antibody technologies. Most applications for laboratory-based were directed at virus with relatively emphasis fungal bacterial pathogens, though there some good other groups pathogens. With advent molecular biology ability compare regions genomic DNA representing conserved sequences, increased an amazing rate all Comparison ITS bacteria, fungi, nematodes proven useful taxonomic purposes. Sequencing genes been used develop PCR-based varying levels specificity viruses, bacteria. Combinations PCR technologies are improve sensitivity avoid problems inhibitors or often found in plants. The application these pathology greatly improved our detect pathogens is increasing understanding of, their ecology epidemiology.