Specific detection of D- and M-isolates of plum pox virus by immunoenzymatic determination of PCR products

摘要: Molecular techniques based on the polymerase chain reaction (PCR) can provide rapid and sensitive diagnosis of plum pox virus (PPV), causal agent devastating 'sharka' disease stone fruit trees. The present study compared routine procedures against a new system, PCR-ELISA (Boehringer Mannheim), which enables immunoenzymatic detection PCR products. results show that this hybridisation system ensures fast more PPV associated with trees herbaceous hosts. Strain-specific capture probes were also designed to identify two major isolates, D M, without subsequent restriction fragment length polymorphism analysis Optimisation all parameters involved in procedure are discussed its advantages reported.