High-performance liquid chromatography of eburnane alkaloids

摘要: Abstract Methods developed for the separation of eburnane alkaloids have been applied solving different analytical problems; plant extracts, mother liquors and pharmaceutical formulations analysed on both reversed-phase silica packings. For determination apovincaminic acid ethyl ester (vinpocetine) in human blood plasma octylsilica was used as stationary phase acetonitrile–0.01 M aqueous ammonium carbonate eluent. quantitation apovincamine can be an internal standard. The detection limit found to 2 ng/ml. To determine corresponding acids derivatives a ion-pair chromatographic method developed.