A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda

摘要: Chitinase, belonging to family 18 glycosyl hydrolase, is a multi-gene and it has many functions. Generation of loss-of-function mutant targeting an interesting gene common way clarify its function based on reverse genetics. In this study, we first reported the immune defense chitinase (EcChi4) in Exopalaemon carinicauda using EcChi4-deletion mutant. EcChi4 was predominantly expressed hepatopancreas upregulated after challenge with Vibrio parahaemolyticus or Aeromonas hydrophila. After knockout CRISPR/Cas9 tool, prawns group had significant higher mortality than those wild-type when were challenged V. A. conclusion, demonstrate E. by performing directed, heritable mutagenesis. future, should be widely applicable as feasible means for editing study important biological questions that cannot easily addressed other decapods.