Coupling SPE on-line pre-enrichment with HPLC and MS/MS for the sensitive detection of multiple allergens in wine

摘要: Abstract Mass spectrometry (MS) represents an essential tool in proteomics studies, the last years also exploited for monitoring allergens contamination food products. Milk and egg are renowned often used as fining agents to promote clarification of wines, therefore any residual amount end-products could represent a menace allergic individuals. In view this, it is paramount importance have at disposal sensitive analytical methods able detect traces milk food. this work we describe upgrade optimization workflow based on use pre-enrichment column coupled with HPLC separation MS/MS detection selective white wine. Two different sample pre-treatments mass cut-off filters or size exclusion cartridges were evaluated compared, before tryptic digestion LC-SRM-MS/MS analysis resulting peptides mixture. The devised UF method peptide on-line enabled reach lowest LODs down 0.036 μg/mL 0.05 μg/mL respectively, proving be most strategy