Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation
作者： Eunjoo Lee , Daeyoung Kim
DOI: 10.1155/2018/6784591
关键词:
摘要: The purpose of this study is to evaluate the effects astaxanthin added freezing buffer on semen parameters, total sperm oxidation stress after postthawing boar sperm, and lipid peroxidation (LPO) which caused by reactive oxygen species (ROS) in membrane. Varying concentrations (0, 10, 50, 100, 500 μM) were used during cryopreservation protect DNA thawed miniature pig sperm. Semen parameter was measured using computer-assisted analysis (CASA) for motility, then ROS rate oxidative determined fluorescence-activated cell sorting (FACS). Sperm motility higher ( ) group than control group. number progressive motile 500 μM In evaluation, had lower intracellular O2 H2O2 viable Yo-Pro-I/HE PI/H2DCFDA staining as revealed flow cytometry groups other groups. As a result, we found that could plasma membrane from free radicals LPO postthawing.
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