Detection of Meloidogyne incognita and Pochonia chlamydosporia by fluorogenic molecular probes
作者： A. Ciancio , A. Loffredo , F. Paradies , C. Turturo , M. Finetti Sialer
DOI: 10.1111/J.1365-2338.2005.00794.X
关键词:
摘要: Fluorescent molecular probes were applied for detection of the plant parasitic nematode Meloidogyne incognita and nematode-egg fungus Pochonia chlamydosporia var. chlamydosporia. A region in M. rDNA including ITS2 was selected amplification recognition with a real-time PCR assay, based on combination three specific motifs, each recognized by fluorescent probe. Similarly, Scorpion probe designed RT-PCR quantification P. c. For this purpose, ITS-2 gene sequenced from number Italian isolates. conserved unique found used. The allowed single juveniles mycelium- or soil-extracted fungal DNA. potentialities procedures are discussed.
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