Regulation of islet β-cell proliferation by prolactin in rat islets

摘要: This study examined the effects of prolactin on β-cell proliferation in pancreatic islet Langerhans. Insulin secretion and were significantly increased from neonatal rat islets cultured for 4 days presence either 500 ng/ml ovine (oPRL) or (rPRL). These could be prevented by including anti-oPRL serum culture media. Although insulin slightly higher during first 24 h exposure to rPRL, maximal response was observed after 6–10 proliferation. The initial mitogenic rPRL occurred limited recruitment nondividing β-cells into cell cycle most daughter cells proceeding directly additional division cycles. Subsequently, effect maintained a rate previously with only one fourth continuing divide. observations are difficult reconcile proposal that pool capable undergoing exists islets. Instead, these suggest individual transiently re-entering dividing infrequently rPRL. In this case, majority would not expected an irreversible G o phase. We also demonstrated occur at normal glucose concentrations affected inhibitors polyamine metabolism. Additional studies should provide important information regulation conditions demand.