Stimulating beta cell replication and improving islet graft function by GPR119 agonists.

摘要: G protein-coupled receptor 119 (GPR119) is predominantly expressed in β cells and intestinal L cells. In this study, we investigated whether oleoylethanolamide (OEA), a GPR119 endogenous ligand, PSN632408, synthetic agonist, can stimulate β-cell replication vitro vivo improve islet graft function diabetic mice. We found that OEA PSN632408 significantly increased numbers of insulin(+)/5-bromo-2'-deoxyuridine (BrdU)(+) cultured mouse islets dose-dependent manner. All recipient mice, given marginal syngeneic transplants with or vehicle, achieved normoglycemia at 4 weeks after transplantation. However, was faster OEA- PSN632408-treated mice than vehicle-treated (P < 0.05). The percentage insulin(+)/BrdU(+) grafts higher 0.01). Our data demonstrated function. Targeting novel therapeutic approach to increase mass by stimulating replication.