Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.

摘要: Lung cancer remains the leading killer around world. It's crucial to identify newer mechanism-based targets effectively manage lung cancer. Annexin A5 (ANXA5) is a protein kinase C inhibitory and calcium dependent phospholipid-binding protein, which may act as an endogenous regulator of various pathophysiological processes. However, its molecular mechanism in poorly understood. This study was designed determine ANXA5 with hope obtain useful information provide new therapeutic target. We used stable isotope dimethyl labeling based quantitative proteomic method differentially expressed proteins NSCLC cell lines after transfection. Out 314 proteins, we identified 26 44 that were down- up-regulated upon modulation, respectively. The IPA analysis revealed glycolysis gluconeogenesis predominant pathways modulated by ANXA5. Multiple central nodes, namely HSPA5, FN1, PDIA6, ENO1, ALDOA, JUP KRT6A appeared occupy regulatory nodes protein-protein networks modulation. Taken together, appears have pleotropic effects, it modulates multiple key signaling pathways, supporting potential usefulness target might insight into