Long Noncoding RNA WEE2-AS1 Plays an Oncogenic Role in Glioblastoma by Functioning as a Molecular Sponge for MicroRNA-520f-3p.

摘要: The long noncoding RNA WEE2 antisense 1 (WEE2-AS1) plays an oncogenic role in hepatocellular carcinoma and triple negative breast cancer progression. In this study, we investigated the expression roles of WEE2-AS1 glioblastoma (GBM). Furthermore, molecular mechanisms behind actions GBM cells were explored detail. was detected using quantitative real-time polymerase chain reaction. evaluated by cell counting kit-8 assay, flow cytometric analysis, Transwell migration invasion assays, tumor xenograft experiments. evidently enhanced tissues lines compared with their normal counterparts. An increased level correlated average diameter, Karnofsky Performance Scale score, shorter overall survival among patients. Functionally, depleted attenuated proliferation, migration, vitro, promoted apoptosis, impaired growth vivo. Mechanistically, functioned as a sponge for microRNA-520f-3p (miR-520f-3p) consequently specificity protein (SP1) cells. A series recovery experiments revealed that inhibition miR-520f-3p upregulation SP1 could partially abrogate influences downregulation on conclusion, can adsorb to increase endogenous expression, thereby facilitating malignancy GBM. Therefore, targeting WEE2-AS1miR-520f-3pSP1 pathway might be promising therapy management future.