Improvements in the method for the electron microscopic localization of arylsulphatase activity
作者： Väinö K. Hopsu-Havu , Antti U. Arstila , Heikki J. Helminen , Hannu O. Kalimo , George G. Glenner
DOI: 10.1007/BF00279874
关键词:
摘要: The optimal conditions for the demonstration of arylsulphatase activity in proximal convoluted tubule cells rat kidney were studied at light and electron microscopic level. 8-hydroxyquinoline sulphate, p-nitrophenyl sulphate 2-hydroxy-5-nitrophenylsulphate used as substrates barium lead capturing ions. effect fixation, ions, substrate concentration pH was biochemically. results these biochemical studies then verified histochemically. Finally a recommended method presented.
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