In vivo molecular mapping of the tumor microenvironment in an azoxymethane-treated mouse model of colon carcinogenesis.

摘要: Background and Objective Development of miniaturized imaging systems with molecular probes enables examination changes leading to initiation progression colorectal cancer in an azoxymethane (AOM)-induced mouse model the disease. Through improved novel studies animal disease models, more effective diagnostic treatment strategies may be developed for clinical translation. We introduce use a multimodal endoscope lavage-delivered fluorescent examine dynamic microenvironment AOM-treated model. Study Design/Materials Methods The is equipped optical coherence tomography (OCT) laser induced fluorescence (LIF) modalities. It used Cy5.5-conjugated antibodies create time-resolved maps colon carcinogenesis. monitored vivo expression over five month period four biomarkers: epithelial growth factor receptor (EGFR), transferrin (TfR), transforming beta 1 (TGFβ1), chemokine (C-X-C motif) 2 (CXCR2). In OCT LIF images were compared multiple time points correlate increases biomarker adenoma development. Results This system uniquely capable tracking time. Increased panel corresponded sites offered predictive utility highlighting prior detectable structural changes. Biomarker also tended increase higher tumor burden rate colon. Conclusion We can dual modality endoscopes study developmental models supplement findings from biopsy tissue harvesting. Lasers Surg. Med. 47:40–49, 2015. © 2014 Wiley Periodicals, Inc.