Time-serial Assessment of Drug Combination Interventions in a Mouse Model of Colorectal Carcinogenesis Using Optical Coherence Tomography.

摘要: Optical coherence tomography (OCT) is a high-resolution, nondestructive imaging modality that enables time-serial assessment of adenoma development in the mouse model colorectal cancer. In this study, OCT was utilized to evaluate effectiveness interventions with experimental antitumor agent α-difluoromethylornithine (DFMO) and nonsteroidal anti-inflammatory drug sulindac during early [chemoprevention (CP)] late stages [chemotherapy (CT)] colon tumorigenesis. Biological endpoints for included OCT-generated tumor number burden. Immunochistochemistry used biochemical [Ki-67, cleaved caspase-3, cyclooxygenase (COX)-2, β-catenin]. K-Ras codon 12 mutations were studied polymerase chain reaction-based technique. We demonstrated significantly correlated histological analysis both burden all groups (P < 0.0001), but allows more accurate full characterization growth rate because its time-serial, nature. DFMO alone or combination suppressed CP setting DFMO-mediated decrease cell proliferation (Ki-67, P 0.001) K-RAS frequency = 0.04). CT setting, DFMO/sulindac effective reducing number, not rate. A COX-2 staining (COX-2, 0.01) confirmed treatment effect. Use enabled repeated, quantitative evaluation burden, allowing changes these parameters be measured as result CT. conclusion, robust minimally invasive method monitoring cancer disease therapies models.