Synergistic Effect of Green Tea Catechins on Cell Growth and Apoptosis Induction in Gastric Carcinoma Cells
作者： Nobuyuki Horie , Naomi Hirabayashi , Yoshie Takahashi , Yuko Miyauchi , Hiroko Taguchi
DOI: 10.1248/BPB.28.574
关键词:
摘要: (−)-Epigallocatechin gallate (EGCG), a major component of green tea catechins, is known to inhibit cell growth and induce apoptosis in variety cultured cells. We examined effects catechins cells derived from human gastric carcinoma. The proliferation four lines (MKN-1, MKN-45, MKN-74 KATO-III) was inhibited with EGCG dose-dependent manner. MKN-45 most efficiently by the treatment (IC50: 40 μM EGCG) among lines, while KATO-III were insensitive 80—150 μM) treatment. In addition, (−)-epicatechin (EC) had synergistic effect on induction treated EGCG; however it little inhibition induced EGCG. To study molecular mechanisms behind EGCG, activity caspases examined. Activity levels caspases-3, -8 -9 elevated EGCG-treated cells, suggesting that these are involved Furthermore, EC specifically canceled catalase treatment, synergism involves extracellular production reactive oxygen species.
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