Detection and genotype analysis of human papillomavirus in non-small cell lung cancer patients

摘要: Although the role of human papillomavirus (HPV) in development uterine cervical cancer is well established, HPV lung carcinogenesis remains controversial. The detection rates DNA are subject to a wide variation from 0 100%. This partly influenced by techniques employed. To elucidate impact infection on parenchyma, we analyzed 100 non-small cell (NSCLC) specimens (39 squamous carcinomas, 50 adenocarcinomas, 5 samples with characteristics both and adenocarcinoma, undifferentiated 1 large carcinoma) region Crete, Greece. Sixteen non-cancerous served as negative controls. was extracted paraffin-embedded tissue sections obtained NSCLC patients. were examined for Real-Time PCR using GP5+/GP6+ primers. Furthermore, HPV-positive subjected genotyping. In contrast absence viral genomes control samples, detected 19 (19%). particular, 4 12 sample 2 HPV-positive. distribution genotypes follows: 16: eight cases (42.1%); 11: three (15.8%); 6: one case (5.2%); 59: 33: two (10.5%); 31: (10.5%) 18: (10.5%). presence tumor provides evidence potential strongly argues additional research this issue.