Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts.

摘要: Previously we reported that nuclear export of both unspliced and spliced murine leukemia virus (MLV) transcripts depends on the factor (NXF1) pathway. Although mRNA complex TREX, which contains Aly/REF, UAP56, THO complex, is involved in NXF1-mediated cellular mRNAs, its contribution to MLV remains poorly understood. Here, studied involvement TREX components transcripts. Depletion but not reduced level viral cytoplasm. Interestingly, depletion components, including THOC5 THOC7, affected only Moreover, RNA immunoprecipitation assay showed transcript interacted with THOC5. These results imply requires addition NXF1, for Given naturally intronless bulk require export, it plausible plays a key role