Direct visualisation of plasmid DNA in individual chromatography adsorbent particles by confocal scanning laser microscopy.
作者： Anders Ljunglöf , Peder Bergvall , Ramagauri Bhikhabhai , Rolf Hjorth
DOI: 10.1016/S0021-9673(99)00386-6
关键词:
摘要: Confocal microscopy was used for the measurement of plasmid DNA adsorbed to individual adsorbent particles intended anion-exchange and triple helix affinity chromatography. Plasmid visualized with fluorescent dye YOYO-1, that forms a highly complex double stranded DNA. images were translated into fluorescence intensity profiles distribution in measured. The results adsorption mainly takes place an outer layer particles. described procedure can also be advantageously demonstrate formation between immobilized oligonucleotides.
elsevier.com
sci-hub.se 参考文章(16)
Vladimir N. Potaman, Valery N. Soyfer, Triple-helical nucleic acids ,(1995)
James Pawley, None, Handbook of Biological Confocal Microscopy ,(1995)
P Wils, V Escriou, A Warnery, F Lacroix, D Lagneaux, M Ollivier, J Crouzet, J-F Mayaux, D Scherman, Efficient purification of plasmid DNA for gene transfer using triple-helix affinity chromatography Gene Therapy. ,vol. 4, pp. 323- 330 ,(1997) , 10.1038/SJ.GT.3300388
Gyan Chandra, Pankaj Patel, Thomas A. Kost, John G. Gray, Large-scale purification of plasmid DNA by fast protein liquid chromatography using a Hi-Load Q Sepharose column. Analytical Biochemistry. ,vol. 203, pp. 169- 172 ,(1992) , 10.1016/0003-2697(92)90060-K
Stephane Bancel, Wei-Shou Hu, Topographical imaging of macroporous microcarriers using laser scanning confocal microscopy Journal of Fermentation and Bioengineering. ,vol. 81, pp. 437- 444 ,(1996) , 10.1016/0922-338X(96)85145-2
Anders Ljunglöf, Jörg Thömmes, Visualising intraparticle protein transport in porous adsorbents by confocal microscopy Journal of Chromatography A. ,vol. 813, pp. 387- 395 ,(1998) , 10.1016/S0021-9673(98)00378-1
Duarte Miguel F Prazeres, Thomas Schluep, Charles Cooney, Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. Journal of Chromatography A. ,vol. 806, pp. 31- 45 ,(1998) , 10.1016/S0021-9673(97)01254-5
Laurence R. Brewer, J. Courtney Davidson, Joseph W. Balch, Anthony V. Carrano, Three-dimensional imaging of DNA fragments during electrophoresis using a confocal detector Electrophoresis. ,vol. 16, pp. 1846- 1850 ,(1995) , 10.1002/ELPS.11501601303
Anders Ljunglöf, Rolf Hjorth, Confocal microscopy as a tool for studying protein adsorption to chromatographic matrices Journal of Chromatography A. ,vol. 743, pp. 75- 83 ,(1996) , 10.1016/0021-9673(96)00290-7
Victoria L. Singer, Laurie J. Jones, Stephen T. Yue, Richard P. Haugland, Characterization of PicoGreen Reagent and Development of a Fluorescence-Based Solution Assay for Double-Stranded DNA Quantitation Analytical Biochemistry. ,vol. 249, pp. 228- 238 ,(1997) , 10.1006/ABIO.1997.2177