Cell compaction influences the regenerative potential of passaged bovine articular chondrocytes in an ex vivo cartilage defect model.

摘要: The loss and degradation of articular cartilage tissue matrix play central roles in the process osteoarthritis (OA). New models for evaluating repair/regeneration are thus great value transferring various culture systems into clinically relevant situations. repair can be better monitored ex vivo than in vitro cell cultures. I have therefore established an defect model prepared from bovine femoral condyles by implantation cells cultured ways, e.g., monolayer-cultured or suspension pellet cultures chondrocytes representing different compactions with variable densities chondrocytes. report that integrin subunit α10 was significantly upregulated suspension-cultured at passage P2 compared P1 (p = 0.0083) (p < 0.05). Suspension-cultured did not promote when implanted pellets: 24.0 ± 0.66% cells, 46.4 ± 2.9% monolayer 127.64 ± 0.90% pellets (p < 0.0001) original volume (percentage defect). Additional cultivation chondrogenesis-promoting growth factors TGF-β1 BMP-2 revealed enhancing effect on all settings. advantage innovation this system over differentiation (e.g., micromass, pellet) assays is possibility examining regeneration environment which embedded within native surrounding site. Such explants might serve as a to mimic clinical