Use of a smooth, resorbable template for delivery of cultured pellets of autologous chondrocytes to articular cartilage defects--preliminary report.

摘要: Background: Autologous chondrocyte transplantation (ACT) is the most commonly used cell-based surgical procedure for repair of articular cartilage defects. The challenges this technique include dedifferentiation chondrocytes following several in vitro passages, invasive means transplantation, and inadequate cell retention leading to washout transplanted cells. To overcome these obstacles, we developed a novel transplanting high-density pellets seeded on prefabricated, resorbable, rigid, 2-dimensional template amenable minimally implantation. Methods: Chondrocytes were obtained from costal New Zealand white rabbits expanded monolayer culture. After 2 suspension was centrifuged total 1 × 106 cells condensed surface resorbable LactoSorb plate (0.5-mm thick, 4-mm diameter). construct incubated 24 hours culture medium before into circular diameter, 0.5-mm deep defects non–weight-bearing part femoral condyle. Control left empty or implanted with alone. Macroscopic histological evaluation performed 4 weeks posttransplantation. Results: Macroscopically, boundaries all demarcated distinguishable adjacent intact cartilage. Regenerative tissue experimental group appeared white, smooth, uniform showing more resemblance hyaline groups revealed absent cartilaginous filled soft, fibrous an irregular surface. Histologically, control fibroinflammatory no evidence continuous chondrocytic regeneration. Cartilage regeneration continuous, layer surrounding plates. Consistently grown only, amount extracellular matrix deposition less than normal Conclusion: We have approach ACT, utilizing carrier. Our study can serve as model further development evaluating its potential role alternative ACT.