Nanometer-Resolved Mapping of Cell-Substrate Distances of Contracting Cardiomyocytes Using Surface Plasmon Resonance Microscopy.

摘要: It has been shown that quantitative measurements of the cell–substrate distance steady cells are possible with scanning surface plasmon resonance microscopy setups in combination an angle resolved analysis. However, accuracy determined distances as well capabilities for investigation cell dynamics remained limited due to assumption a homogeneous refractive index cytosol. Strong spatial or temporal deviations between local and average value can result errors calculated around 100 nm, while was 37 nm. Here, we present acquisition analysis techniques enables measurement contractile study intracellular processes through changes at diffraction limit. By decoupling th...