The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer

摘要: The identification of cancer-associated long non-coding RNAs and the investigation their molecular biological functions are important for understanding biology progression cancer. HOTAIR (HOX transcript antisense intergenic RNA) has been implicated in several cancers; however, its role non-small cell lung cancer (NSCLC) is unknown. aim present study was to examine expression pattern NSCLC evaluate clinical significance tumor progression. Expression analyzed 42 tissues four lines by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Over-expression RNA interference (RNAi) approaches were used investigate HOTAIR. effect on proliferation evaluated MTT colony formation assays, migration invasion transwell assays. Tail vein injection cells metastasis nude mice. Protein levels targets determined western blot analysis. Differences between groups tested using Student’s t-test (two-tailed). highly expressed both samples compared with corresponding normal counterparts. upregulation correlated advanced pathological stage lymph-node metastasis. Moreover, patients high had a relatively poor prognosis. Inhibition RNAi decreased vitro impeded vivo. HOXA5 affected knockdown or over-expression vitro. Our findings indicate that significantly up-regulated tissues, regulates metastasis, partially via down-regulation HOXA5. Thus, may represent new marker prognosis potential therapeutic target intervention.