Antitumor effect and apoptosis induction of Alocasia cucullata (Lour.) G. Don in human gastric cancer cells in vitro and in vivo.

摘要: Alocasia cucullata (Lour.) G. Don was applied in traditional Chinese medicine for the treatment of cancer Southwest area. Its antitumor effect scrutinized vitro and vivo. And first time, mechanism extract A. (EAC) against human gastric cell well examined. To detect most effective fraction, antiproliferation efficacy four fractions (namely derivatives by adding EAC to n-BuOH, petroleum ether, EtOAc water until dissolve fully) five lines were screened MTT assay. Among fractions, IC50s n-BuOH fraction (EAC-B) time-dependent inhibition line (MGC-803) further investigated (MTT assay). In vivo EAC-B examined MGC-803 bearing tumor nude mice. Especially, paper focused on relevant study included cycle distribution (flow cytometry) cyclin D1 expression (RT-PCR western blot), apoptosis (Hoechst 33342 stain flow cytometry), apoptosis-related protein (Akt, p-Akt, ERK, p-ERK, Bcl-2, Bax) blot, caspase3/7 activity showed its cytotoxicity various lines, particularly cells with IC50 value 18.8 μg/mL vitro. Tumor weight significantly reduced study, increased ratio at G0/G1 phase both mRNA level MGC-803. Chromatin condensation also observed. down-regulated p-ERK up-regulated Bax/Bcl-2 ratio. Further, caspase 3/7 activation enhanced as well. This demonstrated that had potent is primarily via arrest pro-apoptosis, including PI-3 K/Akt pathway, ERK activity, stimulated cytochrome C release accompanied an increase may be a potential source novel compounds treatment.