作者: Claudia Grajeda-Iglesias , Oren Rom , Shadi Hamoud , Nina Volkova , Tony Hayek
DOI: 10.1002/BIOF.1415
关键词:
摘要: Whereas atherogenicity of dietary lipids has been largely studied, relatively little is known about the possible contribution amino acids to macrophage foam-cell formation, a hallmark early atherogenesis. Recently, we showed that leucine antiatherogenic properties in model system. In this study, an in-depth investigation role lipid metabolism was conducted by supplementing humans, mice, or cultured macrophages with leucine. Macrophage incubation serum obtained from healthy adults supplemented (5 g/d, 3 weeks) significantly decreased cellular cholesterol mass inhibiting rate biosynthesis and increasing efflux macrophages. Similarly, supplementation C57BL/6 mice (8 resulted content their harvested peritoneal (MPM) relation reduced rate. Studies J774A.1 murine revealed dose-dependently triglyceride mass. Macrophages treated (0.2 mM) attenuated uptake very low-density lipoproteins rate, concurrent down-regulation diacylglycerol acyltransferase-1, key enzyme catalyzing Similar effects were observed when α-ketoisocaproate, metabolite. Finally, both vivo vitro improved mitochondrial respiration ATP production. The above studies, human, macrophages, highlight protective for attenuating formation mechanisms related cholesterol, triglycerides, energy © 2018 BioFactors, 44(3):245-262, 2018.