Leucine supplementation attenuates macrophage foam‐cell formation: Studies in humans, mice, and cultured macrophages

作者: Claudia Grajeda-Iglesias , Oren Rom , Shadi Hamoud , Nina Volkova , Tony Hayek

DOI: 10.1002/BIOF.1415

关键词:

摘要: Whereas atherogenicity of dietary lipids has been largely studied, relatively little is known about the possible contribution amino acids to macrophage foam-cell formation, a hallmark early atherogenesis. Recently, we showed that leucine antiatherogenic properties in model system. In this study, an in-depth investigation role lipid metabolism was conducted by supplementing humans, mice, or cultured macrophages with leucine. Macrophage incubation serum obtained from healthy adults supplemented (5 g/d, 3 weeks) significantly decreased cellular cholesterol mass inhibiting rate biosynthesis and increasing efflux macrophages. Similarly, supplementation C57BL/6 mice (8 resulted content their harvested peritoneal (MPM) relation reduced rate. Studies J774A.1 murine revealed dose-dependently triglyceride mass. Macrophages treated (0.2 mM) attenuated uptake very low-density lipoproteins rate, concurrent down-regulation diacylglycerol acyltransferase-1, key enzyme catalyzing Similar effects were observed when α-ketoisocaproate, metabolite. Finally, both vivo vitro improved mitochondrial respiration ATP production. The above studies, human, macrophages, highlight protective for attenuating formation mechanisms related cholesterol, triglycerides, energy © 2018 BioFactors, 44(3):245-262, 2018.

参考文章(65)
O. Rom, A. Z. Reznick, Z. Keidar, K. Karkabi, D. Aizenbud, Body composition in heavy smokers: comparison of segmental bioelectrical impedance analysis and dual-energy X-ray absorptiometry. Advances in Experimental Medicine and Biology. ,vol. 840, pp. 1- 11 ,(2014) , 10.1007/5584_2014_16
Yohei Tanada, Tetsuo Shioi, Takao Kato, Akira Kawamoto, Junji Okuda, Takeshi Kimura, Branched-chain amino acids ameliorate heart failure with cardiac cachexia in rats Life Sciences. ,vol. 137, pp. 20- 27 ,(2015) , 10.1016/J.LFS.2015.06.021
José Marín-García, The Role of Mitochondria in Atherosclerosis Springer, Boston, MA. pp. 295- 303 ,(2013) , 10.1007/978-1-4614-4599-9_14
Minoru Yamakado, Kenji Nagao, Akira Imaizumi, Mizuki Tani, Akiko Toda, Takayuki Tanaka, Hiroko Jinzu, Hiroshi Miyano, Hiroshi Yamamoto, Takashi Daimon, Katsuhisa Horimoto, Yuko Ishizaka, Plasma Free Amino Acid Profiles Predict Four-Year Risk of Developing Diabetes, Metabolic Syndrome, Dyslipidemia, and Hypertension in Japanese Population Scientific Reports. ,vol. 5, pp. 11918- 11918 ,(2015) , 10.1038/SREP11918
M. Yamakado, T. Tanaka, K. Nagao, Y. Ishizaka, T. Mitushima, M. Tani, A. Toda, E. Toda, M. Okada, H. Miyano, H. Yamamoto, Plasma amino acid profile is associated with visceral fat accumulation in obese Japanese subjects. Clinical obesity. ,vol. 2, pp. 29- 40 ,(2012) , 10.1111/J.1758-8111.2012.00039.X
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Nicholas P. Gannon, Roger A. Vaughan, Leucine-induced anabolic-catabolism: two sides of the same coin Amino Acids. ,vol. 48, pp. 321- 336 ,(2016) , 10.1007/S00726-015-2109-8
William G. Witham, Keith A. Yester, Kenneth R. McGaffin, A high leucine diet mitigates cardiac injury and improves survival after acute myocardial infarction. Metabolism-clinical and Experimental. ,vol. 62, pp. 290- 302 ,(2013) , 10.1016/J.METABOL.2012.07.023