Phenylalanine transport at the human blood-brain barrier. Studies with isolated human brain capillaries.
作者： T B Choi , W M Pardridge
DOI: 10.1016/S0021-9258(19)84595-7
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摘要: The exquisite sensitivity of brain amino acid availability to changes in plasma composition arises from the uniquely high affinity (low Km) blood-brain barrier transport sites as compared cell membrane systems nonbrain tissues. extension this paradigm rats man assumes that Km human is low rat. This hypothesis tested present studies wherein isolated capillaries are used a model system for barrier. Capillaries were obtained autopsy between 20 and 45 h after death yield free adjoining tissue. [3H]Phenylalanine into human, rabbit, or rat capillary was characterized by two saturable nonsaturable component. values phenylalanine via averaged 0.26 +/- 0.08 22.3 7.1 microM five subjects. These provide first evidence very (Km = microM) neutral at barrier, it hypothesized selectively localized side results also show virtually identical both
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