Lung cancer chorioallantoic membrance M-CDX (membrane-based cell-derived xenograft) model and preparation method

摘要: The invention relates to the field of life science and particularly a lung cancer chorioallantoic membrance M-CDX (membrane-based cell-derived xenograft) model preparation method. Thelung is formed by transplanting xenogeneic cells membrance. method comprises steps: an embryo egg incubated in incubator with temperature 35 DEG 40 humidity 60% 80% for 2 4 days, window 1 cm*1 cm cut open at top end egg; (0.5 8)*10 are incorporated into extracellular matrix prepare cell suspension, suspension dripped silicone gel ring pre-placed on membrane, incubatingfor 3 days 50% 65% carried out, microscopic examination shooting out. built chick as transplantation object, carrier load tumor membrane rich blood vessels, andcompared traditional model, disclosed has unique advantages short experimental period, low feeding cost easy operation; operating instruments devices required simple use, price low.