Mature mainstream TCR alpha beta+CD4+ thymocytes expressing L-selectin mediate "active tolerance" in the nonobese diabetic mouse.

摘要: Pathogenic autoreactive T lymphocytes are mediators of spontaneous insulin-dependent diabetes in nonobese diabetic (NOD) mice. This is demonstrated by their capacity to transfer into syngeneic immunoincompetent recipients. In addition, especially prediabetic NOD mice, peripheral CD4 + were identified that highly effective, conventional mixing cotransfer experiments, at preventing disease transfer. The present data demonstrate mature heat-stable Ag − TCRαβ CD8 thymocytes from mice also express this inhibitory capacity. Selection using an L-selectin (CD62L)-specific Ab showed CD62L thymocytes, emerging the mainstream differentiation pathway, concentrate ability regulate effectors. Compared with significantly lower numbers sufficient achieve efficient inhibition NOD- scid protective was potentiated following vitro culture presence IL-7. contrast, enriched class I-restricted NK cells, unable influence Identical results obtained have been cultured for 4 days These support active role a thymus-derived subset controls pathogenic autoimmune