Bioanalytical chromatographic methods for the determination of catechol-O-methyltransferase inhibitors in rodents and human samples: A review

摘要: In the past years, it has been recognised that levodopa therapy may be improved with therapeutic regimens including a catechol-O-methyltransferase (COMT) inhibitor. At present time, tolcapone and entacapone are only two COMT inhibitors available in market. However, further under development for Parkinson's disease, namely nebicapone opicapone (formerly known as BIA 9-1067). addition, nitecapone, another well-known inhibitor, is also preclinical but neuropathic pain. Since 1990s different liquid chromatography methods have developed validated to quantify tolcapone, entacapone, some metabolites biological samples, particularly plasma samples obtained from rodent human species. These bioanalytical primarily used support pharmacokinetic assays such non-clinical clinical studies. As these hydrophobic groups their chemical structures, reversed-phase major approach determination of compounds, especially high-performance coupled ultraviolet detection (HPLC-UV), electrochemical (HPLC-ECD) mass spectrometry (HPLC-MS). Regarding sample preparation, traditional liquid-liquid extraction (LLE) solid-phase (SPE) were most widely procedures analytes interest prior analysis samples. Thus, this review aimed gather, first sufficient background information about chromatographic which already applied metabolites. Moreover, aspects perspective addressed.