Annular PIP3 accumulation controls actin architecture and modulates cytotoxicity at the immunological synapse
作者： Audrey Le Floc’h , Yoshihiko Tanaka , Niels S. Bantilan , Guillaume Voisinne , Grégoire Altan-Bonnet
DOI: 10.1084/JEM.20131324
关键词:
摘要: The immunological synapse formed by a T lymphocyte on the surface of target cell contains peripheral ring filamentous actin (F-actin) that promotes adhesion and facilitates directional secretion cytokines cytolytic factors. We show growth maintenance this F-actin is dictated annular accumulation phosphatidylinositol trisphosphate (PIP3) in synaptic membrane. PIP3 functions context recruiting exchange factor Dock2 to periphery synapse, where it drives polymerization through Rho-family GTPase Rac. also generated class IA phosphoinositide 3-kinases associate with receptor microclusters are activated Ras. Perturbations inhibit or promote PIP3-dependent remodeling dramatically affect cytotoxicity, demonstrating functional importance pathway. These results reveal how cells use lipid-based signaling control architecture modulate effector responses.
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