Transcript Expression in Saccharomyces cerevisiae at High Salinity

摘要: Transcript expression of Saccharomyces cerevisiae at high salinity was determined by microarray analysis 6144 open reading frames (ORFs). From cells grown in 1 m NaCl for 10, 30, and 90 min, changes transcript abundance >2-fold were classified. Salinity-induced ORFs increased over time: 107 (10 min), 243 (30 354 (90 min). Up-regulated, functionally unknown from 17 to 149 this period. Expression patterns similar early, with 67% up-regulated transcripts after 10 min identical those 30 min. The profile revealed different (identities 13% 22%, respectively). Nucleotide amino acid metabolism exemplified the earliest responses salinity, followed related intracellular transport, protein synthesis, destination. Transcripts energy production throughout time course respiration-associated strongly induced Highly expressed known stress-induced genes, detoxification-related responses, transporters major facilitator superfamily, reserves, nitrogen sulfur compounds, lipid, fatty acid/isoprenoid biosynthesis. We chose severe stress conditions monitor essential biochemical mechanisms. In mutant, Deltagpd1/gpd2, lacking glycerol biosynthesis, response magnified a partially set ORFs.