Various cell types in human atherosclerotic lesions express ICAM-1. Further immunocytochemical and immunochemical studies employing monoclonal antibody 10F3.

摘要: The specificity of monoclonal antibody 10F3, generated to smooth muscle cells isolated from fetal human aorta, has been further explored in a series biological, biochemical, and immunocytochemical studies. In the first assay, it was found that 10F3 could inhibit aggregation phytohemagglutinin (PHA)-induced lymphocytes manner comparable RR1/1, an anti-intercellular adhesion molecule 1 (ICAM-1) antibody. immunoprecipitation experiments followed by one-dimensional gel electrophoresis, both RR1/1 immunoprecipitated 90 kd proteins, with results suggesting two antibodies recognized different epitopes same molecule. A studies on atherosclerotic lesions performed; using single-labeling techniques, 10F3-positive were vessel wall virtually all specimens fatty streaks fibrous plaques. Using double-labeling macrophages found; however, there also significant number non-smooth muscle, nonmacrophage cells. These demonstrate identifies ICAM-1, this protein is expressed variety cell types lesions. ICAM-1 may represent developmentally regulated but not adult mesenchymal cells, can be re-expressed pathologic processes such as atherosclerosis.