Metabolic cooperation in cell culture: Studies of the mechanisms of cell interaction

摘要: Metabolic cooperation is a form of cell communication in which the mutant phenotype enzyme deficient cells, as determined by incorporation labeled substrates, corrected culture contact with normal cells. Previous studies showed that metabolic between and hypoxanthine phosphoribosyl transferase cells (HPRT−) was result transfer product enzyme, nucleotide or derivative, from to rather than informational macromolecules leading synthesis enzyme. In present study nature mechanisms involved these interactions were investigated. Effective observed within one hour contact. Modifications extracellular environment including changes osmolarity, concentration sodium divalent ions failed interfere significantly transfer. Changes shape induced cyclic nucleotides, hormones urea also did not affect communication. Cytochalasin B dissociates microfilaments binds membranes reduced while colcemide microtubules had little effect. Enzymatic oxidation iodination surface structures abolished cooperation. The subcellular localization label donor important determining efficiency efficient when radioactive primarily located nucleus inefficient if cytoplasmic. Cell lines previously classified “non-communicating” because they lack gap junctions, ionic coupling shown communicate incubated substrates for 20 hours 3. more generalized phenomenon among appreciated.