Characterization of intestinal cnf1+ Escherichia coli from weaned pigs

作者: István Tóth , Eric Oswald , Jacques G. Mainil , Mohamed Awad-Masalmeh , Béla Nagy

DOI: 10.1016/S1438-4221(00)80019-3

关键词:

摘要: Escherichia coli isolated from 204 cases of porcine postweaning diarrhoea were tested by PCR for the genes cytotoxic necrotic factors (CNF) and cytolethal dystending toxin (CDT). Selected strains also examined presence papC-, sfa-, f17-, f18-, afa-specific sequences encoding P, S, F17, F18 fimbriae afimbrial adhesins. A 5.9% (12/204) had cnf1 gene, two them cdt gene as well. Further six cdt+ detected which cnf-negative. Most cnf1+ belonged to serogroups O2, O6, O8, O54 characteristic necrotoxic E. (NTEC) humans. All possessed P or S both, but negative F4, Results suggest that these enteric isolates may have entero- and/or uropathogenic significance in weaned pigs, zoonotic potential

参考文章(26)
C Le Bouguénec, Y Bertin, AFA and F17 adhesins produced by pathogenic Escherichia coli strains in domestic animals. Veterinary Research. ,vol. 30, pp. 317- 342 ,(1999)
Jacques Mainil, F. Hérault, E. Oswald, E. Jacquemin, Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family. Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire. ,vol. 61, pp. 193- 199 ,(1997)
M J Brian, M Frosolono, B E Murray, A Miranda, E L Lopez, H F Gomez, T G Cleary, Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome. Journal of Clinical Microbiology. ,vol. 30, pp. 1801- 1806 ,(1992) , 10.1128/JCM.30.7.1801-1806.1992
J De Rycke, E A González, J Blanco, E Oswald, M Blanco, R Boivin, Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. Journal of Clinical Microbiology. ,vol. 28, pp. 694- 699 ,(1990) , 10.1128/JCM.28.4.694-699.1990
P. Rippinger, H.U. Bertschinger, H. Imberechts, B. Nagy, I. Sorg, M. Stamm, P. Wild, W. Wittig, Designations F18ab and F18ac for the related fimbrial types F107, 2134P and 8813 of Escherichia coli isolated from porcine postweaning diarrhoea and from oedema disease. Veterinary Microbiology. ,vol. 45, pp. 281- 295 ,(1995) , 10.1016/0378-1135(94)00141-I
Benito Guimarães de Brito, Domingos da Silva Leite, Rosa Elisa C Linhares, Marilda Carlos Vidotto, None, Virulence-associated factors of uropathogenic Escherichia coli strains isolated from pigs. Veterinary Microbiology. ,vol. 65, pp. 123- 132 ,(1999) , 10.1016/S0378-1135(98)00277-6