The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response

摘要: FUS, EWS and TAF15 are structurally similar multifunctional proteins that were first discovered upon characterization of fusion oncogenes in human sarcomas leukemias. The belong to the FET (previously TET) family RNA-binding implicated central cellular processes such as regulation gene expression, maintenance genomic integrity mRNA/microRNA processing. In present study, we investigated expression localization multiple tissues cell types. expressed both distinct overlapping patterns tissues. three showed almost ubiquitous nuclear FUS addition cytoplasm most Cytoplasmic was more rarely detected seen mainly secretory Furthermore, downregulated differentiating embryonic stem cells, during induced differentiation neuroblastoma cells absent terminally differentiated melanocytes cardiac muscle cells. targeted stress granules by heat shock oxidative required its domain for this translocation. spreading initiation centers adhering Our results point cell-specific functions rather than housekeeping roles inferred from earlier studies. suggests activities translational conditions. Roles response, control adhesion may explain frequent involvement cancer.