Diagnosis of treponemal co-infection in HIV-infected West Africans.

摘要: Objectives  To evaluate the performance of two enzyme immunoassays (EIA), Murex and ICE, Determine TP point-of-care test (POCT) in diagnosing treponemal infection (syphilis or yaws) patients attending a large HIV clinic Ghana; to determine prevalence co-infections; characterise demographic clinical features with infection. Methods  Samples were tested EIAs rapid plasma reagin (RPR), then POCT reference assays for Treponema pallidum active past infection. Sensitivity specificity each assay calculated characteristics compared. Data collected from case notes retrospectively. Results  Overall, 45/284 patient samples (14.8%, 95% CI, 11.1–19.4%) particle agglutination (TPPA) positive, these, 27 (64.3%) RPR positive 4 (8.9%) IgM positive. Both showed high sensitivities specificities identifying although was less reliable. Clinical syphilis yaws rarely identified TPPA-positive suggesting most had previous late latent Treatment various intercurrent infections using short courses antibiotics against T. pallidum common clinic. Conclusions  A proportion this HIV-infected cohort evidence as well practical effective at co-infection setting. alone unreliable co-infection, however might be useful some settings where treponemal-specific are unaffordable. Objectifs:  Evaluer la de deux tests immunoenzymatiques et ICE le ‘Determine (POCT)’ dans diagnostic l’infection treponemique ou pian) chez les frequentant une grande clinique du VIH au determiner coinfection caracteriser caracteristiques demographiques cliniques des infectes. Methodes:  Les echantillons ont ete testes avec EIA rapide reagine plasmatique puis pour (TPPA), afin precedente. La sensibilite specificite chaque calculees comparees. donnees collectees retrospectivement partir cas sur patients. Resultats:  Dans l’ensemble, IC95%: etaient positifs TPPA ceux-ci, treponemiques. tous demontre sensibilites specificites elevees identifier l’infection, alors que etait moins fiable. pian rarement identifiees TPPA, suggerant plupart avaient precedentes latentes tardives. Des traitements d’infections intercurrentes diverses utilisant regimes d’antibiotiques courte duree, actifs contre T. pallidumetait courants clinique. Conclusions:  Une forte cette cohorte infectee par montre signes d’infection treponemique. ainsi pratiques efficaces diagnostiquer ce contexte. Le seul n’etait pas fiable coinfections treponemiques actives, mais pourrait etre utile certains contextes specifiques sont inabordables. Objetivos:  Evaluar el desempeno dos inmunoensayos enzimaticos (IEE), e y prueba rapida situ en diagnostico infeccion por treponema (sifilis o pian), pacientes una gran clinica para determinar prevalencia coinfecciones con treponema; las caracteristicas demograficas clinicas los infeccion. Metodos:  Con muestras se realizaron pruebas IEEs Prueba Rapida Reagina despues referencia T. pallidum, fin infecciones activas pasadas. Se calcularon sensibilidad especificidad cada ensayo compararon pacientes. Los datos recogieron forma retrospectiva historias pacientes. Resultados:  En general, IC, eran TP-PA positivas, estas positivas treponema. Tanto como mostraron alta identificar infeccion, aunque menos confiable. Las sifilis rara vez identificadas positivos, sugiriendo mayoria habian tenido previas latente tardias. El tratamiento varias concurrentes, utilizando tratamientos cortos antibioticos activos frente era practica comun clinica. Conclusiones:  Una proporcion esta infectada mostro evidencia practicas efectivas coinfeccion treponema, podria ser util emplazamientos especificas son inasequibles.