Development and application of a novel screening method and experimental use of the mutant bacterial strain Clostridium beijerinckii NCIMB 8052 for production of butanol via fermentation of fresh cassava

摘要: Classic chemical mutagenesis has a demonstrated potential to create strain of Clostridium with improved fermentation performance for obtaining high butanol yield. However, screening methods select the desired bacterial phenotype from large populations are not currently feasible or readily available. In this paper, simple, novel method target strains by using trypan blue dye as an indicator in solid starch media detect mutant bacterium was devised. A well performing beijerinckii no. 15 obtained method. It derived wild-type C. NCIMB 8052 N-methyl-N′-nitro-N-nitrosoguanidine (NTG) mutagenesis, and its fermentative capability producing investigated, hydrolysate fresh cassava other substrates. The produced maximum yield 15.06 g L−1 23.78 total solvents, when substrate given simple pretreatment at 121 °C 1 h. For substrates, highest yields: 20.67 glucose, 17.96 liquefied dextrin, 13.59 corn powder (P ≤ 0.05) were also achieved. Experimental trials showed that had significantly greater ability, high-yield hydrolysates. Further research overall resulted improvement enzymatic activity 15's α-amylase 0.05). Also, protein structure predicted amino acid mutation sites analyzed. Results revealed these acids may have somehow thus, led more butanol. Our will contribute efforts develop produce