The role of crustins in Litopenaeus vannamei in response to infection with shrimp pathogens: An in vivo approach

摘要: Crustin antimicrobial peptides, identified in crustaceans, are hypothesized to have both and protease inhibitor activity based on their primary structure vitro assays. In this study, a reverse genetic approach was utilized test the hypothesis that crustins vivo response bacterial fungal challenge. Injection of double-stranded RNA specific 120-bp region LvABP1, one most prominent crustin isoforms, yielded significant reduction expression mRNA protein within hemocytes. To role shrimp immune response, RNAi first used suppress animals were subsequently injected with low pathogenic doses either Vibrio penaeicida or Fusarium oxysporum. A increase mortality crustin-depleted observed infected V. as compared controls, whereas no change following infection F.