Generation of human cardiomyocytes: a differentiation protocol from feeder-free human induced pluripotent stem cells.

摘要: In order to investigate the events driving heart development and determine molecular mechanisms leading myocardial diseases in humans, it is essential first generate functional human cardiomyocytes (CMs). The use of these cells drug discovery toxicology studies would also be highly beneficial, allowing new pharmacological molecules for treatment cardiac disorders validated pre-clinically on origin. Of possible sources CMs, induced pluripotent stem (iPS) are among most promising, as they can derived directly from readily accessible patient tissue possess an intrinsic capacity give rise all cell types body 1. Several methods have been proposed differentiating iPS into ranging classical embryoid bodies (EBs) aggregation approach chemically defined protocols 2,3. this article we propose EBs-based protocol show how method employed efficiently CM-like feeder-free cells.