The expression, lamin-dependent localization and RNAi depletion phenotype for emerin in C. elegans.

摘要: Emerin belongs to the LEM-domain family of nuclear membrane proteins, which are conserved in metazoans from C. elegans humans. Loss emerin in humans causes X-linked form Emery-Dreifuss muscular dystrophy (EDMD), but disease mechanism is not understood. We have begun address the function emerin , a genetically tractable nematode. The gene ( emr-1 ) C. elegans . detect Ce-emerin protein envelopes all cell types except sperm, and find that co-immunoprecipitates with Ce-lamin embryo lysates. show for first time any organism that nuclear lamins are essential envelope localization emerin during early development. further four other types nuclear envelope including fellow Ce-MAN1, as well as Ce-lamin, UNC-84 nucleoporins do depend on their localization. This result suggests organize or localize only lamin (B-type) expressed also analyzed RNAi phenotype resulting loss in under laboratory growth conditions, found no detectable throughout propose an appropriate system which study molecular mechanisms vivo.