作者: Mami HIWASA , Hirohide KOHNO , Tetsuro TOGARI , Kentaro OKABE , Yutaka FUKUI
DOI: 10.1262/JRD.20062
关键词:
摘要: The present study aimed to investigate the fertility of ewes artificially inseminated with three different methods using a synthetic semen extender, AndroMed. artificial insemination (AI) were cervical AI fresh-diluted or frozen-diluted at observed estrus, and an intrauterine frozen-thawed semen. A total 80 treated controlled internal drug release (CIDR) containing 0.3 g progesterone per device for 12 days. In Experiment 1 (26 Suffolk ewes), superovulation was induced 20 mg follicle-stimulating hormone 250 IU equine chorionic gonadotropin (eCG) two days one day before CIDR removal, respectively, during non-breeding season. 2 (54 crossbred intramuscular injection 500 eCG administered removal synchronize estrus ovulation breeding 1, deposited into orifice after detection, performed by laparoscopy fixed-time basis without detection. Embryos recovered uterine flushing 6 AI, rates recovered, fertilized (cleaved) ova embryos morula blastocyst stage compared among methods. 2, pregnancy compared. not significantly (52.5-56.7%). rate (81.0%) laparoscopic higher (25.5%) (3.5%) semen, but (17.6%) lower than that (69.2%). yielding groups (44.4, 11.1 62.5% semen). intrauterinally (72.2%) those cervically (5.5%) (0.0%) results showed acceptable fertilization could be obtained extender (AndroMed), sufficient either fresh frozen