Chimeric synthetic peptides from the envelope (gp46) and the transmembrane (gp21) glycoproteins for the detection of antibodies to human T-cell leukemia virus type II.
作者： Milenen Hernández Marin , Patricia Castellanos Pentón , Yadaris Márquez Bocalandro , Lilliam Pozo Peña , Janet Díaz Navarro
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摘要: Abstract Two chimeric synthetic peptides incorporating immunodominant sequences from HTLV-II virus were synthesized. Monomeric P2 and P3 represent transmembrane protein (gp21) envelope (gp46) of the virus. The peptide is a gp21 (370–396) sequence gp46 (178–205) sequence. Those arranged in way that permits one to obtain different combinations (P2-GG-P3 P3-GG-P2), separated by two glycine residues as spacer arms. antigenic activity these was evaluated UltramicroEnzyme-linked immunosorbent assay (UMELISA) using panels anti-HTLV-II-positive sera ( n = 11), anti-HTLV-I/II-positive 2), HTLV-positive (untypeable) serum samples anti-HTLV-I-positive 22), while specificity with anti-HIV-positive 19) healthy blood donors 30). efficacy solid-phase immunoassays compared monomeric mixture peptides. Higher sensitivity observed for Q5 assay. results may be related higher adsorption capacity solid surface epitope accessibility antibodies. This would very useful diagnostic.
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