Identification of a Novel Guanine Nucleotide Exchange Factor for the Rho GTPase

摘要: The Rho GTPase promotes proliferation and cytoskeletal rearrangements in mammalian cells. To understand the regulation of Rho, it is important to characterize guanine nucleotide exchange factors (GEFs), which stimulate dissociation GDP subsequent binding GTP. Using as an affinity ligand, we have isolated a 115-kDa protein (p115-RhoGEF) that binds specifically nucleotide-depleted state. A full-length cDNA encoding p115-RhoGEF was isolated, its product, exhibited sequence homology Dbl Lbc, catalyzed for GTP on not Rac, Cdc42, or Ras GTPases. capable regulating cell proliferation, determined by ability induce transformation NIH 3T3 Northern Western analysis suggests ubiquitously expressed. These results indicate may be general regulator associated cellular phenotypes.