Immunohistochemical staining for S100P, SMAD4, and IMP3 on cell block preparations is sensitive and highly specific for pancreatic ductal adenocarcinoma.

摘要: Introduction The diagnosis of pancreatic ductal adenocarcinoma (PDA) on endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) material is often challenging. An immunohistochemical (IHC) panel may help establish the PDA in cases limited by sample size or ambiguous cytology. S100P, IMP3, and SMAD4 are 3 IHC markers that have shown promise as individual for never been tested together a panel. In this study, we evaluated combined efficacy detection PDA. Materials Methods staining was performed cell blocks (CBs) procured from EUS-FNA procedures. cohort included CBs were diagnostic (n = 35), suspicious but nondiagnostic 2), well with benign epithelium 12) reactive 18). A positive result IMP3 S100P defined moderate strong >10% cells. Complete lack nuclear considered result—any negative result. Results Two marker panels almost always more specific than markers. Positivity at least 2 sensitive (91.89%) highly (100%) Conclusions composed Future studies should evaluate atypical cases.